Difference between revisions of "Part:BBa K3183010"
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[[File:T--Oxford--Results-ldh.png|thumb|right|430px|'''Figure 2:''' - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. <i> Error bars represent Standard error of the mean. n=3 </i>]] | [[File:T--Oxford--Results-ldh.png|thumb|right|430px|'''Figure 2:''' - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. <i> Error bars represent Standard error of the mean. n=3 </i>]] | ||
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Revision as of 16:15, 21 October 2019
mClover3 Fluorescent Protein, Codon Optimized for L. reuteri
mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.
Figure 2: - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. Error bars represent Standard error of the mean. n=3
Fluorescence wavelengths
Bajar et al report the following excitation and emission data for mClover3 -
- Excitation max - 506nm
- Emission max - 518nm
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.