Difference between revisions of "Part:BBa K3046001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part is | + | This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites. |
===Source=== | ===Source=== |
Revision as of 16:10, 21 October 2019
PLEAPglaA_2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 50
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
Source
This part is created on the basis of the upstream region of the glaA ORF (Systematic ORF name: An03g06550) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model