Difference between revisions of "Part:BBa K2971004"
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Expression was induced in 2&#x25; arabinose </br>
 
Expression was induced in 2&#x25; arabinose </br>
 
From left to right &#58;Uninduced <i>E.coli</i> with empty expression vector, Induced <i>E.coli</i> with empty expression vector, induced <i>E.coli</i> with <i>crtEBI</i> expression vector, uninduced <i>E.coli</i> with <i>crtEBI</i> expression vector.
 
From left to right &#58;Uninduced <i>E.coli</i> with empty expression vector, Induced <i>E.coli</i> with empty expression vector, induced <i>E.coli</i> with <i>crtEBI</i> expression vector, uninduced <i>E.coli</i> with <i>crtEBI</i> expression vector.
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<img style="width:40% !important;" src="https://2019.igem.org/wiki/images/4/4e/T--UiOslo_Norway--our%26pBad.jpg">
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<strong>Figure 1: Expression of crtEBI in Escherichia coli (BL21)</strong></br>
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Expression was induced in 2&#x25; arabinose </br>
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From left to right &#58;Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.
 
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Revision as of 18:27, 21 October 2019


crtEBI under pBad promotor

This composite part contains the genes crtE, crtB and crtI combined into a single reading frame to produce lycopene when expressed in Escherichia coli. The genes express geranylgeranyl diphosphate synthase (GGPPS), phytoene synthase (CrtB) and phytoene desaturase (CrtI) respectively. GGPPS and CrtB produce the saturated compound phytoene, which is then desaturated by CrtI into the conjugated compound lycopene. When the part was expressed in E.coli, the pellet of the spun down expression culture acquired a pink color.

Figure 1: Expression of pBAD-crtEBI in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.

Figure 1: Expression of crtEBI in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 4181
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2854
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2093
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1295
    Illegal SapI site found at 961