Difference between revisions of "Part:BBa K3100001"

Revision as of 17:15, 21 October 2019

T7 Promoter T7-1
PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7. From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it: https://2019.igem.org/Team:SCUT_China/Design

Figure. The strength of PT7 1-48

References:

Ghodasara A, Voigt CA. Balancing gene expression without library construction via a reusable sRNA pool. Nucleic Acids Res. 2017;45(13):8116–8127. doi:10.1093/nar/gkx530

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3100001 short</partinfo><br>
 PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7.
-<p>From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it.https://2019.igem.org/Team:SCUT_China/Design</p>
+From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it: https://2019.igem.org/Team:SCUT_China/Design
 <center>[[File:T - SCUT China- T7 promoter 1-48.jpeg|500px]]<br>
 Figure. The strength of PT7 1-48</center>