Difference between revisions of "Part:BBa K2992002"

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===Characterisation===
 
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We made three <i>BotR</i>-expression modules comprising of the <i>BotR</i> sigma factor gene under the control of the native <i>BotR</i> promotor,an inducible system and one with no promotor. See the[https://2019.igem.org/Team:Nottingham/Results results page] for more information <br><br><br>
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This is a basic part that we used in many experiments to produce the sigma factor to induce the expression of acetone, GusA or FAST.
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We made three <i>BotR</i>-expression modules comprising of the <i>BotR</i> sigma factor gene under the control of the native <i>BotR</i> promotor,an inducible system and one with no promotor. See the[https://2019.igem.org/Team:Nottingham/Results results page] for more information.
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https://2019.igem.org/wiki/images/5/5e/T--Nottingham--Basic4.png
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https://2019.igem.org/wiki/images/6/6b/T--Nottingham--Basic3.png
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Revision as of 16:46, 21 October 2019


botR gene from C. botulinum

botR encodes a positive transcriptional regulator of C. botulinum neurotoxin production which functions as an alternative sigma factor.

Usage and Biology

BotR regulates the the expression of boutlinum neruotoxin and non-toxic associated proteins during the late exponential and early stationary growth phases. We have integrated botR into the genome of C. sporogenes to generate a reporter strain to validate the concept of using volatile reporters to predict the production of botulinum neurotoxin following food manufacturing processes.

Characterisation

This is a basic part that we used in many experiments to produce the sigma factor to induce the expression of acetone, GusA or FAST. We made three BotR-expression modules comprising of the BotR sigma factor gene under the control of the native BotR promotor,an inducible system and one with no promotor. See theresults page for more information.


T--Nottingham--Basic4.png
T--Nottingham--Basic3.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.

Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.