Difference between revisions of "Part:BBa K2996503"
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| − | <center><b> | + | <center><b>Fig. 1 Overlap PCR product of C-luciferase and DBD.(Lane1:overlap PCR product;Lane 2:2000bp ladder)</i> </center> |
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| − | <center><b> | + | <center><b>Fig. 2.</b> <i>The working pattern of BBa_K2996503.</i> </center> |
Revision as of 23:49, 21 October 2019
The part is aimed to create a fused protein of C-luciferase and zincfinger
This part is made up of part BBa_K2996502 and part BBa_K2996501,which produces a fused protein of C-luciferase and zincfinger.We used overlapping PCR to produce the DNA fragment.The fused protein is aimed to bring the C-luciferase to the site of target sequence of zincfinger. The product of this part will bind to the binding site of DNA binding domain,thus bringing the C-luciferase near the "lure" sequence in our project.The dCas9 fused with N-luciferase will bind to "lure" sequence once the off-target incidence takes place.When the two parts of luciferase get near,they will make the whole enzyme.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 846
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 302
Illegal AgeI site found at 441 - 1000COMPATIBLE WITH RFC[1000]
