Difference between revisions of "Part:BBa K3038004:Design"
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Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. | Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. | ||
doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610 | doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610 | ||
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Latest revision as of 15:24, 21 October 2019
Acyl CoA oxydase : Mlut11700_N-ter C-myc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1682
Illegal XhoI site found at 1925 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1816
Illegal BsaI.rc site found at 2047
Illegal SapI site found at 172
Design Notes
C MYC Tags has been added on the Nterminal part of the sequence.
The sequence is flanked with the sequence of EcoRI and XbaI on the 5'end and the SpeI and PstI on the 3' end.
Design of the Mlut_11700 gene with the Geneious Software.
The design show restriction enzymes like EcoRI, XbaI, SpeI and PstI. The gene includes pBAD promoter and its terminater. Finally C-myc tag is present in N-term.
The RFC10 standard was followed.
The GC content has been optimized for the synthesis by IDT
Source
Sequence synthezized by IDT
References
Engineering of Bacterial Methyl Ketone Synthesis for Biofuels. Ee-Been Goh,a,c Edward E. K. Baidoo,a,c Jay D. Keasling,a,c,d and Harry R. Beller. Appl Environ Microbiol. 2012 Jan; 78(1): 70–80. doi: 10.1128/AEM.06785-11. PMCID: PMC3255637. PMID: 22038610