Difference between revisions of "Part:BBa J10053:Experience"
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===User Reviews=== | ===User Reviews=== | ||
| + | = Lambert_GA 2019 Characterization = | ||
| + | ==Characterization of Medium Promoter (BBa_J22016) and Weak RBS (BBa_B0031)== | ||
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Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | ||
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| − | The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.</ | + | <center>[[File:T--Lambert GA--tuning4b.png|800px]]</center> |
| + | <br> | ||
| + | <center><i>Figure 1. The highlighted points in yellow represent BBa_J10053 (medium promoter/ weak RBS) compared to BBa_J10054(medium promoter/ strong RBS) and BBa_J10055(medium promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter. </i></center> | ||
| + | <br> | ||
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| + | <center>[[File:T--Lambert GA--tuning4a.png|800px]]</center> | ||
| + | <br> | ||
| + | <center><i>Figure 2. The highlighted points represent the BBa_J10053 composite part (medium promoter/ weak RBS). As the promoter strength increases, the expression increases as well.</i></center> | ||
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| + | <center>The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.</center> | ||
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Revision as of 15:17, 21 October 2019
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Applications of BBa_J10053
User Reviews
Lambert_GA 2019 Characterization
Characterization of Medium Promoter (BBa_J22016) and Weak RBS (BBa_B0031)
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10053 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10054 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
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