Difference between revisions of "Part:BBa J10050:Experience"
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<center><i>Figure 1. The highlighted points in yellow represent BBa_J10050 (weak promoter/ weak RBS) compared to BBa_J10051(weak promoter/ strong RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.</i></center>
 
<center><i>Figure 1. The highlighted points in yellow represent BBa_J10050 (weak promoter/ weak RBS) compared to BBa_J10051(weak promoter/ strong RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.</i></center>
  
 
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<center><i>Figure 2. The highlighted points represent the BBa_J10050 composite part (weak promoter/ weak RBS). As the promoter strength increases, the expression increases as well.</center>
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<center><i>Figure 2. The highlighted points represent the BBa_J10050 composite part (weak promoter/ weak RBS). As the promoter strength increases, the expression increases as well.</center></i>
  
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The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.</i>
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The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.
  
  

Revision as of 14:41, 21 October 2019


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Applications of BBa_J10050

User Reviews

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Lambert_GA 2019 Characterization

Characterization of weak promoter (BBa_J23113) and weak RBS (BBa_B0031)

Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10050 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10051 and BBa_J10052, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.


T--Lambert GA--tuning1a.png



Figure 1. The highlighted points in yellow represent BBa_J10050 (weak promoter/ weak RBS) compared to BBa_J10051(weak promoter/ strong RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.


T--Lambert GA--tuning1b.png



Figure 2. The highlighted points represent the BBa_J10050 composite part (weak promoter/ weak RBS). As the promoter strength increases, the expression increases as well.</center>

The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.













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