Difference between revisions of "Part:BBa K3117020"
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Part BBa_K3117020 is a single chain variable fragment (scFv) of the anti-CD3 antibody. This fragment was used to generate a fusion protein to create a bispecific antibody on the one hand driected against CD3, on the other part against GPA33. | Part BBa_K3117020 is a single chain variable fragment (scFv) of the anti-CD3 antibody. This fragment was used to generate a fusion protein to create a bispecific antibody on the one hand driected against CD3, on the other part against GPA33. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | CD3 is co-expressed with the T cell receptor on T cells. Binding leads to downstream signaling cascades and activation of the T cell. | + | CD3 is co-expressed with the T cell receptor on T cells. Binding leads to downstream signaling cascades and activation of the T cell[2]. |
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Revision as of 22:31, 21 October 2019
scFv VH against CD3 codon optimized for CHO expression
Part BBa_K3117020 is a single chain variable fragment (scFv) of the anti-CD3 antibody. This fragment was used to generate a fusion protein to create a bispecific antibody on the one hand driected against CD3, on the other part against GPA33.
Usage and Biology
CD3 is co-expressed with the T cell receptor on T cells. Binding leads to downstream signaling cascades and activation of the T cell[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 142
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]