Difference between revisions of "Part:BBa K2992033"
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===Characterisation=== | ===Characterisation=== | ||
| − | + | This part is the ribosome binding site for the translation of the <i> add </i> gene from <i> C. acetobutylicum </i>. This was integrated in to the larger composite parts:[https://parts.igem.org/Part:BBa_K2992028 BBa_K2992028], [https://parts.igem.org/Part:BBa_K2992029 BBa_K2992029], [https://parts.igem.org/Part:BBa_K2992030 BBa_K2992030], [https://parts.igem.org/Part:BBa_K2992034 BBa_K2992034], [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035] and [https://parts.igem.org/Part:BBa_K2992036 BBa_K2992036]. See See [https://2019.igem.org/Team:Nottingham/Results results page]for more | |
Revision as of 20:29, 21 October 2019
RBS region for the adc gene from C. acetobutylicum.
RBS region for the adc gene from C. acetobutylicum.
Usage and Biology
This parts entry represents the RBS for the acetoacetate decarboxylase adc gene in C. acetobutylicum. The gene product of adc catalyses the decarboxylation of acetoacetate into acetone and CO2. adc is a central component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes.
Characterisation
This part is the ribosome binding site for the translation of the add gene from C. acetobutylicum . This was integrated in to the larger composite parts:BBa_K2992028, BBa_K2992029, BBa_K2992030, BBa_K2992034, BBa_K2992035 and BBa_K2992036. See See results pagefor more
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Cornillot, E., Croux, C. and Soucaille, P. (1997). Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome. Journal of Bacteriology, 179(23), pp.7426-7434.