Difference between revisions of "Part:BBa K3117006"
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<partinfo>BBa_K3117006 short</partinfo>
 
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The Igk leader is a signaling sequence, directing the protein into the secretory pathway.
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The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011).  
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The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011). By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest.
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The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection.
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<partinfo>BBa_K3117006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3117006 SequenceAndFeatures</partinfo>
  
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By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest.
 +
The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection.
  
 
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Revision as of 14:33, 21 October 2019


Igk leader sequence

The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest. The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection.