Difference between revisions of "Part:BBa K3117006"
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The Igk leader is a signaling sequence, directing the protein into the secretory pathway. | The Igk leader is a signaling sequence, directing the protein into the secretory pathway. | ||
| + | The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011). By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest. | ||
| + | The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 14:01, 21 October 2019
Igk leader sequence
The Igk leader is a signaling sequence, directing the protein into the secretory pathway. The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011). By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest. The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]