Difference between revisions of "Part:BBa K2992022"

Revision as of 13:50, 21 October 2019

5-UTR containing RBS for bgaR from C. perfringens

Component of the divergent PbgaR -PbgaL promoter predicted to regulate bgaR in C. perfringens.

Usage and Biology

The BgaR-BgaL system of C. perfringens comprises the transcriptional regulator BgaR belonging to the AraC-family and the β-galactosidase BgaL which are transcribed in a regulated fashion from the divergent PgaR -PgaL promoter. The BgaR-BgaL system regulates the expression of carbohydrate metabolic genes in response to lactose concentrations (Hartman and Melville 2011). This parts entry represents the RBS and 5’-UTR predicted to regulate bgaR. Our group has recently utlised the BgaRL regulatory system in order to generate a tightly regulate inducible system for CRISPR-Cas mutagenesis in the genus Clostridium (Cañadas et al., 2019). In our project, we use the PgaR -PgaL regulatory system comprised of the divergent promoter and associated 5’-UTRs in conjunction with their cognate transcriptional regulator bgaR (hyperlinks and descriptions) to drive the expression of our volatile and FAST reporter genes in an inducible fashion. Doing so helps us fulfil our goal of generating reporter strains for the prediction of botulinum neurotoxin production following food manufacture.

Characterisation

Data incoming

Sequence and Features

References

Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019].

Hartman, A., Liu, H. and Melville, S. (2010). Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression inClostridium perfringens. Applied and Environmental Microbiology, 77(2), pp.471-478.