Difference between revisions of "Part:BBa K2976007"
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<p>According to the design of our plasmid, the sequence of lamp2 and HA tag was combined for expressing fusion protein, since the HA tag was characterized by western blot, the protein lamp2 was also proved to be expressed together with HA tag. | <p>According to the design of our plasmid, the sequence of lamp2 and HA tag was combined for expressing fusion protein, since the HA tag was characterized by western blot, the protein lamp2 was also proved to be expressed together with HA tag. | ||
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| + | [[File:T--CPU CHINA--HA tag-WB.tiff|600px|left|Figure 2: Western blot result of exosome protein.]] | ||
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Revision as of 13:56, 21 October 2019
Lamp 2
Usage and Biology
Lysosomal associated membrane protein 2 (Lamp 2) is a member of a family of membrane glycoproteins, which is highly expressed on the surface of exosomes. Much research has indicated that peptides fused to the N terminus of Lamp2 could be effectively displayed on the outer surface of exosomes. In 2019 CPU_CHINA project, we display PD-L1 targeting peptide on outer surface of exosomes and adopt this targeting exosome system to deliver hsa-let-7f into Mtb-infected macrophages specifically.
Characteristics
According to the design of our plasmid, the sequence of lamp2 and HA tag was combined for expressing fusion protein, since the HA tag was characterized by western blot, the protein lamp2 was also proved to be expressed together with HA tag.
File:T--CPU CHINA--HA tag-WB.tiff
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]