Difference between revisions of "Part:BBa K2999001"
| Line 4: | Line 4: | ||
This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding ''Pseudomonas aeruginosa'' PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site. | This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding ''Pseudomonas aeruginosa'' PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site. | ||
| − | For compatibility, 254<sup>th<sup><br/> nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine). | + | For compatibility, 254<sup>th</sup><br/> nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine). |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:46, 21 October 2019
RBS+PslG
This part is mainly used for the extracellular polysaccharide hydrolase PslG encoding Pseudomonas aeruginosa PAO1 biofilm and the hydrolysis of such extracellular polysaccharides. The first 50 bp of the sequence is its ribosome binding site.
For compatibility, 254th
nucleotide G was changed to A (same as leucine) and 1358 nucleotide G to A (same as leucine).
Usage and Biology
Please click:BBa_K2999009
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 356
Illegal XhoI site found at 360 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 660
Illegal NgoMIV site found at 849
Illegal NgoMIV site found at 857 - 1000COMPATIBLE WITH RFC[1000]