Difference between revisions of "Part:BBa K3286104"

Latest revision as of 14:54, 21 October 2019

Expression cassette for small proteins in E. coli BL21DE3

Use this part, containing a T7 promoter (BBa_K1614000), Bicistronic Design (BCD) system [1](BBa_M36516), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (BBa_K3286103) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.

[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 493
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 191

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286104 short</partinfo>
-Use this part, containing a T7 promoter (<partinfo>BBa_K1614000</partinfo>), Bicistronic Design (BCD) system (<partinfo>BBa_M36516</partinfo>), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (<partinfo>BBa_K3286103</partinfo>) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.
+Use this part, containing a T7 promoter (<partinfo>BBa_K1614000</partinfo>), Bicistronic Design (BCD) system [1](<partinfo>BBa_M36516</partinfo>), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (<partinfo>BBa_K3286103</partinfo>) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.
+[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
 <!-- Add more about the biology of this part here