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Revision as of 15:40, 21 October 2019
Synthetic guide RNA sequence 2 for botR integration
Synthetic guide RNA sequence 2 for the integration of several botR constructs at the pyrE locus of the C. sporogenes genome
Usage and Biology
Synthetic guide RNA sequence 2 for the use of CRISPR-Cas9 for genomic integration of various botR constructs in the generation of our volatile reporter strains for predicting neurotoxin production following food manufacture. Genomic integration of these constructs was at the pyrE locus of C. sporogenes.
Characterisation
This basic part was used to construct our
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.
Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.