Difference between revisions of "Part:BBa K2992007"

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===Characterisation===
 
===Characterisation===
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This is a basic part utalised in a composite part characterised in our acetone producing reporters:[https://parts.igem.org/Part:BBa_K2992028 BBa_K2992028], [https://parts.igem.org/Part:BBa_K2992029 BBa_K2992029], [https://parts.igem.org/Part:BBa_K2992030 BBa_K2992030], [https://parts.igem.org/Part:BBa_K2992034 BBa_K2992034], [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035], [https://parts.igem.org/Part:BBa_K2992036 BBa_K2992036]. This part was used as a promoter which functions in both the Gram-negative E. coli and the Gram-positive Clostridium <i>sporogenens</i>. More information can be found on our [https://2019.igem.org/Team:Nottingham/Results Results Page]. <br><br>
  
 
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Revision as of 15:36, 21 October 2019


intergenic region between ctfA and ctfB from C. botulinum

The A and B sub-units of the CtfAB complex are encoded in an operon on the genome of C. botulinum. This part entry represents the intergenic region immediately downstream of the stop codon for ctfA and upstream of the start codon for ctfB. This region also contains part of the RBS for ctfB.


Usage and Biology

The CtfAB complex is central to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. the CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

This is a basic part utalised in a composite part characterised in our acetone producing reporters:BBa_K2992028, BBa_K2992029, BBa_K2992030, BBa_K2992034, BBa_K2992035, BBa_K2992036. This part was used as a promoter which functions in both the Gram-negative E. coli and the Gram-positive Clostridium sporogenens. More information can be found on our Results Page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Vital, M., Howe, A. and Tiedje, J. (2014). Revealing the Bacterial Butyrate Synthesis Pathways by Analyzing (Meta)genomic Data. mBio, 5(2).