Difference between revisions of "Part:BBa K3100001"
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PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7. | PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7. | ||
From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. <a href="https://2019.igem.org/Team:SCUT_China/Design">How our VerProS pool work you can learn from it.</a><br> | From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. <a href="https://2019.igem.org/Team:SCUT_China/Design">How our VerProS pool work you can learn from it.</a><br> | ||
Revision as of 12:56, 21 October 2019
T7 Promoter T7-1
PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7.
From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. <a href="https://2019.igem.org/Team:SCUT_China/Design">How our VerProS pool work you can learn from it.</a>
Figure. The strength of PT7 1-48References:
Ghodasara A, Voigt CA. Balancing gene expression without library construction via a reusable sRNA pool. Nucleic Acids Res. 2017;45(13):8116–8127. doi:10.1093/nar/gkx530
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]