Difference between revisions of "Part:BBa K2913021"
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Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil (5-FU), which has an extreme damage to the bacteria. | Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil (5-FU), which has an extreme damage to the bacteria. | ||
| − | We added gradient of 5-FC culturing the bacteria and | + | We added gradient of 5-FC culturing the bacteria and monitored their growth. |
[[Image:T--NEFU_China--parts--CD1.png|600px|thumb|left|Fig.1 Growth of <i>E.coli</i> at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB media (containing 50µg/ml Amp and Str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (<i>E.coli</i>) using T7 to express CD. B. The bacteria (<i>E.coli</i>) can`t express CD.]] | [[Image:T--NEFU_China--parts--CD1.png|600px|thumb|left|Fig.1 Growth of <i>E.coli</i> at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB media (containing 50µg/ml Amp and Str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (<i>E.coli</i>) using T7 to express CD. B. The bacteria (<i>E.coli</i>) can`t express CD.]] | ||
Revision as of 12:29, 21 October 2019
Cytosine deaminase
An enzyme that oxidizes 5-fluorouracil to 5-fluorouracil
Usage and Biology
result
Cytosine deaminase (CD) is the enzyme that can transform cytosine into uracil. CD’s function is to convert nontoxic 5-Flucytosine (5-FC) into toxic 5-fluorouracil (5-FU), which has an extreme damage to the bacteria.
We added gradient of 5-FC culturing the bacteria and monitored their growth.
Fig.1 Growth of E.coli at different concentration of 5-FC. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging : 2μl bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB media (containing 50µg/ml Amp and Str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria (E.coli) using T7 to express CD. B. The bacteria (E.coli) can`t express CD.
As a positive control, we added gradient of 5-FU culturing the bacteria and monitored their growth.
Fig.2 Growth of bacteria at different concentrations of 5-FU. Foster the bacteria (keeping the quantity of the bacteria same before 1% passaging: 2ul bacteria, OD=3.5) with a concentration gradient of 5-FC in the LB media (containing 50 µg/ml Amp and Str): 500mg/l, 250mg/l, 100mg/l, 50mg/l, 25mg/l, 0mg/l. A. The bacteria using T7 to express CD. B. The original bacteria that without the CD expression plasmid.
For more details about the part, please visit NEFU_China 2019 wiki.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1140
- 1000COMPATIBLE WITH RFC[1000]