Difference between revisions of "Part:BBa K3173007"

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Linker for Tango like GPCR assays.
 
Linker for Tango like GPCR assays.
 
Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI.  
 
Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI.  
Before coupling upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment.
+
Before coupling, upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:26, 21 October 2019


v2-tail with efficient TCS and SV 40 NLS

Linker for Tango like GPCR assays. Couple it to GPCR´s for improved arrestin binding plus mediated TEV Protease cleavage to release downstream POI. Before coupling, upstream GPCR should be checked for arrestin recruitment and/or typical Phosphorylation motifs to allow such recruitment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]