Difference between revisions of "Part:BBa K2054001"

(→‎Usage and Biology)
(→‎Usage and Biology)
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                       showing that the purified ssDNAs produced from plasmid could work as per their design and  
 
                       showing that the purified ssDNAs produced from plasmid could work as per their design and  
 
                       function well. Details of our experimental characterization could be found on
 
                       function well. Details of our experimental characterization could be found on
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                     https://2019.igem.org/Team:HK_SKHLPSS/Characterization
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                     https://2019.igem.org/Team:HK_SKHLPSS/Characterization.
 
                       </li>
 
                       </li>
  
−
 
+
<img src="https://2019.igem.org/wiki/images/2/2a/T--HK_SKHLPSS--HKU_assay1.png" alt="p1" style="width:75%"/>
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<img src="https://2019.igem.org/wiki/images/4/48/T--HK_SKHLPSS--HKU_assay2.png" alt="p1" style="width:75%"/>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 10:33, 21 October 2019


Oligo 1

This will be use to build our desired nanostructure. This ssDNA contains also split G-quadruplex sequences that allow the oligo itself to function as an DNAzyme under specific conditions.


Usage and Biology

  • Group: Team: HK_SKHLPSS, 2019
  • Author: Tse Cheong Tat
  • Summary: The peroxidase activity of the nano-structure formed from parts BBa_K2054001and BBa_K2054005 were measured. It was found that the nano-structure formed from these two parts had a high specificity in detecting the target sequence, showing that the purified ssDNAs produced from plasmid could work as per their design and function well. Details of our experimental characterization could be found on https://2019.igem.org/Team:HK_SKHLPSS/Characterization.

    • <img src="T--HK_SKHLPSS--HKU_assay1.png" alt="p1" style="width:75%"/> <img src="T--HK_SKHLPSS--HKU_assay2.png" alt="p1" style="width:75%"/>

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 7
      Illegal NheI site found at 30
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]