Difference between revisions of "Part:BBa K3061002"
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It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.
 
It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.
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<h1>1. biology</h1>
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<p>Channelrhodopsin 2（ChR2） is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc（BBa_K3061010链接）around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):<p>
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<img  src="https://static.igem.org/mediawiki/parts/3/3d/T--DUT_China_B--BBa_K3061002.png">
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<center><p >Figure 1. Results of SignalP 4.1 server</p></center>
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<partinfo>BBa_K3061002 parameters</partinfo>
 
<partinfo>BBa_K3061002 parameters</partinfo>
 
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<h3>1. biology</h3>
 
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<p>Channelrhodopsin 2（ChR2） is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc（BBa_K3061010链接）around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):<p>
 
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<img  src="https://static.igem.org/mediawiki/parts/3/3d/T--DUT_China_B--BBa_K3061002.png">
 
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<center><p >Figure 1. Results of SignalP 4.1 server</p></center>
 
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Revision as of 14:25, 21 October 2019


Sp, signal peptide for Chlamydomonas reinhardtti

It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.

1. biology

Channelrhodopsin 2（ChR2） is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc（BBa_K3061010链接）around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):

Figure 1. Results of SignalP 4.1 server

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]