Difference between revisions of "Part:BBa K3286215"
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<span class=‘h3bb’>Sequence and Features</span>
 
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===Functional Parameters===
 
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Revision as of 09:23, 21 October 2019

kaiABC Operon

The folowwing composite parts contains the cyanobacterial circadian oscillator core genes. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts [1]. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.

  1. [1]Cohen, SE., and Golden, SS. (2015) Circadian Rhythms in Cyanobacteria. Microbiol. Biol. Rev. 79(4), 373-385.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 264
    Illegal XhoI site found at 331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 394