Difference between revisions of "Part:BBa K3137013"
 
Line 9: Line 9:
 
The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA(([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002])) and a green fluorescent protein gene <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]). In burst period (about 20 min), there is an inducible promoter PglcF([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]), and a red fluorescent protein gene <i>mCherry</i>([https://parts.igem.org/Part:BBa_K3137004# BBa_K3137004]).
 
The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA(([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002])) and a green fluorescent protein gene <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]). In burst period (about 20 min), there is an inducible promoter PglcF([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]), and a red fluorescent protein gene <i>mCherry</i>([https://parts.igem.org/Part:BBa_K3137004# BBa_K3137004]).
  
−
When E. coli is infected by a phage, report gene circuits are induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002]) will induce the bacteria to expressthe green fluorescent protein gene <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]).
+
When <i>E. coli</i> is infected by a phage, report gene circuit is induced to express. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible promoter PputA([https://parts.igem.org/Part:BBa_K3137002# BBa_K3137002]) will induce the bacteria to expressthe green fluorescent protein gene <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]).
  
 
[[Image:PputA.png|500px|thumb|center|Green fluorescence of <i>E.coli</i> before and after phage infection]]
 
[[Image:PputA.png|500px|thumb|center|Green fluorescence of <i>E.coli</i> before and after phage infection]]

Latest revision as of 09:12, 21 October 2019


report circuit

Report circuit.png

Usage and Biology

The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA((BBa_K3137002)) and a green fluorescent protein gene gfp(BBa_K3137011). In burst period (about 20 min), there is an inducible promoter PglcF(BBa_K3137003), and a red fluorescent protein gene mCherry(BBa_K3137004).

When E. coli is infected by a phage, report gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA(BBa_K3137002) will induce the bacteria to expressthe green fluorescent protein gene gfp(BBa_K3137011).

Green fluorescence of E.coli before and after phage infection

If the resistant proteins successfully resist phage infection, the inducible promoter PglcF(BBa_K3137003) will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF(BBa_K3137003) will activate the red fluorescent protein gene mCherry. so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.

Red fluorescence of E.coli before and after phage infection

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 1535