Difference between revisions of "Part:BBa K3137004"
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===Usage and Biology===
 
===Usage and Biology===
  
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When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF will induce expression of the red fluorescent protein gene mCherry([https://parts.igem.org/Part:BBa_K3137004# BBa_K3137004]), which will make the bacteria display red fluorescence.
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When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF([https://parts.igem.org/Part:BBa_K3137003# BBa_K3137003]) will induce expression of the red fluorescent protein gene mCherry, which will make the bacteria display red fluorescence.
  
 
[[Image:PglcF.png|500px|thumb|center|Characterization of PglcF]]
 
[[Image:PglcF.png|500px|thumb|center|Characterization of PglcF]]

Revision as of 08:48, 21 October 2019


mCherry

The mCherry can express red fluorescent protein and it is used as a marker gene in the process of reporting the number of cells.

Usage and Biology

When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF(BBa_K3137003) will induce expression of the red fluorescent protein gene mCherry, which will make the bacteria display red fluorescence.

Characterization of PglcF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]