Difference between revisions of "Part:BBa K3113009"

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L7Ae

L7Ae is a 50S ribosomal protein found in Archea. It is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D, box C'/D' sRNAs. This RNA-binding protein can be used as an adapter for RNA binding in the extracellular vesicles.

Usage

The informational readout of ALiVE is RNA. To load a specific RNA into the vesicles we fused it to RNA-motifs which are bound specifically by RNA binding proteins. Two combinations of binding protein and motif were tested. One was the archaeal RNA binding protein L7Ae which binds to the k-turns in the box C/D snoRNP.

Biology

The archaeal L7Ae and eukaryotic 15.5kD protein homologs are members of the L7Ae/15.5kD protein family that characteristically recognize K-turn motifs found in both archaeal and eukaryotic RNAs. In Archaea, the L7Ae protein uniquely binds the K-loop motif found in box C/D.^[1] The k-turn is a ubiquitous structural motif in RNA forming a very tight kink in the axis of helical RNA that plays an important role in many aspects of RNA function. L7Ae is a member of a superfamily of proteins that bind k-turns in RNA, stabilizing the tightly kinked conformation. They are extremely widespread and are important in the assembly of RNA–protein complexes central to translation, splicing and site-specific RNA modification. ^[2]

Characterization

Sequence and Features

References

1. ↑ Gagnon, Keith T et al. “Signature amino acids enable the archaeal L7Ae box C/D RNP core protein to recognize and bind the K-loop RNA motif.” RNA (New York, N.Y.) vol. 16,1 (2010): 79-90. doi:10.1261/rna.1692310
2. ↑ Lilley, D. M. J. (2019). "The L7Ae proteins mediate a widespread and highly functional protein–RNA interaction." The Biochemist 41(2): 40-44.