Difference between revisions of "Part:BBa K103006"

Revision as of 16:29, 27 October 2008

OmpA outer membrane protein A fused to linker; displays proteins on cell surface

One of our basic bricks used to create fusions attached to outer membrane

This part uses OmpA (outer membrane protein A [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]) fragment (AAs 46-159) N-terminally fused with lipoprotein signal peptide fragment (AAs 1-9)
Proteins fused to Ompa-link are presented on cell's surface
Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners
In our project Ompa-link is used as outer membrane anchor for our selection system
This brick contains our nonstandard restriction sites (NdeI and SacI) that allow 'scarless' cloning and easy creation of translation fusions

Sequence and Features

Assembly Compatibility:

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 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-*OmpA is outer membrane protein from E. coli [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
+*This part uses OmpA (outer membrane protein A [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]) fragment (AAs 46-159) N-terminally fused with lipoprotein signal peptide fragment (AAs 1-9)
 *Proteins fused to Ompa-link are presented on cell's surface
 *Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners