Difference between revisions of "Part:BBa K103004"
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[[Image:Z_spa.jpg]] | [[Image:Z_spa.jpg]] | ||
− | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003). | + | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003)[http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]. Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003). |
*Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | *Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) |
Revision as of 17:28, 24 October 2008
protein ZSPA-1
Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003)[http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]. Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003).
- ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
- We have used ZSPA-1 protein fusions with BBa_K103019 or BBa_K103020 as a prey in our 'hunter and prey' selection system
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]