Difference between revisions of "Part:BBa K103003"
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<partinfo>BBa_K103003 short</partinfo> | <partinfo>BBa_K103003 short</partinfo> | ||
− | B domain of Staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of Z<sub>SPA-1</sub>. | + | B domain of Staphylococcal protein A[http://www.ncbi.nlm.nih.gov/pubmed/7236608?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum], which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of Z<sub>SPA-1</sub>. |
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Revision as of 17:32, 24 October 2008
B domain of Staphylococcal protein A
B domain of Staphylococcal protein A[http://www.ncbi.nlm.nih.gov/pubmed/7236608?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum], which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of ZSPA-1.
Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]
Usage and Biology
- Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
- We have used this protein as hunter fused with BBa_K103017 or BBa_K103018 in our 'hunter and prey' selection system
- This part uses our BamHI/SacI/NdeI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 91
Illegal BamHI site found at 9 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]