Difference between revisions of "Part:BBa K103003"
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*Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance). | *Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance). | ||
*We have used this protein as hunter fused with [[Part:BBa_K103017 | BBa_K103017]] or [[Part:BBa_K103018 | BBa_K103018]] in our 'hunter and prey' selection system | *We have used this protein as hunter fused with [[Part:BBa_K103017 | BBa_K103017]] or [[Part:BBa_K103018 | BBa_K103018]] in our 'hunter and prey' selection system | ||
| − | + | *This part uses our BamHI/SacI/NdeI fusion cloning substandard | |
Revision as of 17:17, 24 October 2008
B domain of Staphylococcal protein A
B domain of Staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G, it is also strong binder of ZSPA-1.
Structure of B domain of Staphylococcal protein A [http://www.rcsb.org/pdb/explore.do?structureId=1BDD]
Usage and Biology
- Protein A is the famous protein from Staphylococcus aureus which binds to constant fragments of IgG antibodies and has many uses in molecular biology (TapTag for instance).
- We have used this protein as hunter fused with BBa_K103017 or BBa_K103018 in our 'hunter and prey' selection system
- This part uses our BamHI/SacI/NdeI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 91
Illegal BamHI site found at 9 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]