Difference between revisions of "Part:BBa K3031015:Design"
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===Design Notes=== | ===Design Notes=== | ||
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments. | Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments. | ||
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"https://2019.igem.org/wiki/images/9/9a/T--SUIS_Shanghai--ironQSassay.png" | "https://2019.igem.org/wiki/images/9/9a/T--SUIS_Shanghai--ironQSassay.png" | ||
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IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine) | IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine) | ||
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===Source=== | ===Source=== | ||
Revision as of 06:16, 21 October 2019
LuxI with FUR box located in the -10 region
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.
"
"
IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)
Source
BioBrick sequences.
References
Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.
Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.