Difference between revisions of "Part:BBa K3031015:Design"
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Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments. | Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments. | ||
| − | [[File: | + | [[File:T--SUIS Shanghai--ironQSassay.png|frame|center|'''OxyR ([https://parts.igem.org/BBa_K1104200 BBa_K1104200]) ''']] |
===Source=== | ===Source=== | ||
Revision as of 06:06, 21 October 2019
LuxI with FUR box located in the -10 region
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.
Source
BioBrick sequences.
References
Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.
Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.