Difference between revisions of "Part:BBa K3139014"

(Usage and Biology)
Line 7: Line 7:
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
In the anti-plasmodium system of NAU-2019, the amount of both TEV protease ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012 BBa_K3139012]) and anti-plasmodium fusion protein ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013 BBa_K3139013]) should be kept at a certain proportion to avoid a waste of cell resources.
+
In the anti-plasmodium system of NAU-2019, the amount of both TEV protease[https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012 BBa_K3139012] and anti-plasmodium fusion protein[https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013 BBa_K3139013] should be kept at a certain proportion to avoid a waste of cell resources.
 
If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.
 
If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.
 
By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model.  
 
By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model.  

Revision as of 04:29, 21 October 2019


J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010 anti-plasmodium system


Usage and Biology

In the anti-plasmodium system of NAU-2019, the amount of both TEV proteaseBBa_K3139012 and anti-plasmodium fusion proteinBBa_K3139013 should be kept at a certain proportion to avoid a waste of cell resources. If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2232
    Illegal NheI site found at 2255
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2582
    Illegal SapI.rc site found at 2930