Difference between revisions of "Part:BBa K3139014"
(→Usage and Biology) |
|||
| Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here--> | <!-- Add more about the biology of this part here--> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | In the anti-plasmodium system of NAU-2019, the amount of both TEV protease | + | In the anti-plasmodium system of NAU-2019, the amount of both TEV protease[https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139012 BBa_K3139012] and anti-plasmodium fusion protein[https://parts.igem.org/wiki/index.php?title=Part:BBa_K3139013 BBa_K3139013] should be kept at a certain proportion to avoid a waste of cell resources. |
If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. | If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. | ||
By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model. | By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model. | ||
Revision as of 04:29, 21 October 2019
J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010
anti-plasmodium system
Usage and Biology
In the anti-plasmodium system of NAU-2019, the amount of both TEV proteaseBBa_K3139012 and anti-plasmodium fusion proteinBBa_K3139013 should be kept at a certain proportion to avoid a waste of cell resources. If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2232
Illegal NheI site found at 2255 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 444
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2582
Illegal SapI.rc site found at 2930