Difference between revisions of "Part:BBa K079026"
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<partinfo>BBa_K079026 short</partinfo> | <partinfo>BBa_K079026 short</partinfo> | ||
| − | The GFP reporter protein was put under the control of the BBa_J23118 costitutive promoter. The reporter sequence was spaced from the promoter by the addition of the BBa_C0012 sequence without the RBS. This construct was design as an expression control plasmid. | + | The GFP reporter protein was put under the control of the BBa_J23118 costitutive promoter. The reporter sequence was spaced from the promoter by the addition of the BBa_C0012 sequence without the RBS. This construct was design as an expression control plasmid for the BBa_K079020 circuit. |
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Latest revision as of 19:17, 29 October 2008
GFP reporter protein and LacI gene sequence under the control of J23118 constitutive promoter
The GFP reporter protein was put under the control of the BBa_J23118 costitutive promoter. The reporter sequence was spaced from the promoter by the addition of the BBa_C0012 sequence without the RBS. This construct was design as an expression control plasmid for the BBa_K079020 circuit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1864