Difference between revisions of "Part:BBa K108030:Design"
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===References=== | ===References=== | ||
− | 1. | + | 1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500. |
− | 2. | + | 2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209. |
− | 3. | + | 3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246. |
Revision as of 15:14, 24 October 2008
TARS-LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Design Notes
...
Source
LacI Operator sequence was designed according to reference 1.
TARS sequence was designed according to reference 2.
LacI and T7 terminator was cloned from pET-15b(Novagen).
LVA sequence was designed according to reference 3.
References
1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500.
2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.
3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.