Difference between revisions of "Part:BBa K3187028"
Jaminersss (Talk | contribs) |
PeterGockel (Talk | contribs) |
||
| Line 16: | Line 16: | ||
<tr> | <tr> | ||
<td><b>Base pairs</b></td> | <td><b>Base pairs</b></td> | ||
| − | <td> | + | <td>450</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 25: | Line 25: | ||
<td><b>Origin</b></td> | <td><b>Origin</b></td> | ||
<td><i>Staphylococcus aureus</i>, synthetic</td> | <td><i>Staphylococcus aureus</i>, synthetic</td> | ||
| − | |||
| − | |||
| − | |||
| − | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 02:26, 21 October 2019
Sortase A7M (Ca2+-independent variant)
Profile
| Name | Sortase A7M |
| Base pairs | 450 |
| Molecular weight | 17.85 kDa |
| Origin | Staphylococcus aureus, synthetic |
| Properties | Ca2+-independent, transpeptidase, linking sorting motif LPXTG to poly-glycine Tag |
Usage and Biology
Methods
Results
References
- ↑ Hee-Jin Jeong, Gita C. Abhiraman, Craig M. Story, Jessica R. Ingram, Stephanie K. Dougan, Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling, PLOS ONE, 2017 [1]
- ↑ Hongyuan Mao, Scott A. Hart, Amy Schink, and Brian A. Pollok, Sortase-Mediated Protein Ligation: A New Method for Protein Engineering, JACS, 2004 [2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]