Difference between revisions of "Part:BBa J10050"
Line 4: | Line 4: | ||
Designed and built by Natalie Kuldell for BioBuilder teacher's kit. Weak constitutive promoter, weak RBS and the lacZa.GFP fusion protein. | Designed and built by Natalie Kuldell for BioBuilder teacher's kit. Weak constitutive promoter, weak RBS and the lacZa.GFP fusion protein. | ||
− | |||
− | |||
− | |||
<!-- --> | <!-- --> | ||
Line 12: | Line 9: | ||
<partinfo>BBa_J10050 SequenceAndFeatures</partinfo> | <partinfo>BBa_J10050 SequenceAndFeatures</partinfo> | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | ==Lambert_GA 2019 Characterization== | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 02:50, 21 October 2019
weak promoter + weak RBS + lacZa.GFP fusion
Designed and built by Natalie Kuldell for BioBuilder teacher's kit. Weak constitutive promoter, weak RBS and the lacZa.GFP fusion protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 941