Difference between revisions of "Part:BBa K3183020"
Line 3: | Line 3: | ||
<partinfo>BBa_K3183020 short</partinfo> | <partinfo>BBa_K3183020 short</partinfo> | ||
− | This part is used for composite parts. It separated protein coding regions inside the operon and enables independent translation. | + | This part is used for composite parts. It separated protein coding regions inside the operon and enables independent translation. A high-efficiency ribosome binding site is also located inside this part. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 17: | Line 17: | ||
<partinfo>BBa_K3183020 parameters</partinfo> | <partinfo>BBa_K3183020 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | |||
+ | ===Use by Team Oxford 2019=== | ||
+ | |||
+ | This part was used inside our construct intended to attempt recombinant expression of AgrB and AgrD from <i>C. difficile</i> in <i>E. coli</i>. This part is <partinfo>BBa_K3183202</partinfo>. | ||
+ | |||
+ | ===References=== |
Latest revision as of 15:51, 21 October 2019
Operon Spacer for E. coli
This part is used for composite parts. It separated protein coding regions inside the operon and enables independent translation. A high-efficiency ribosome binding site is also located inside this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Use by Team Oxford 2019
This part was used inside our construct intended to attempt recombinant expression of AgrB and AgrD from C. difficile in E. coli. This part is BBa_K3183202.