Difference between revisions of "Part:BBa K3165042"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | CcaSR is a green-light activated two-component signal transduction system (TCS) that controls the expression of phycobilisome linker protein CpcG2 in Synechocystis sp. PCC6803 complementary chromatic adaptation pathway. The naturally occurring CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. Upon activation with green light, the CcaS is autophosphorylated to a red-absorbing conformation that undergoes phosphotransfer to the response regulator CcaR leading to the activation of the | + | CcaSR is a green-light activated two-component signal transduction system (TCS) that controls the expression of phycobilisome linker protein CpcG2 in Synechocystis sp. PCC6803 complementary chromatic adaptation pathway. The naturally occurring CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. Upon activation with green light, the CcaS is autophosphorylated to a red-absorbing conformation that undergoes phosphotransfer to the response regulator CcaR leading to the activation of the pCpcG2 promoter initiating transcription of the gene of interest.<br> |
In this gene construct, the T7 phage protein Gp2 is used as the gene of interest which has a bacteriostatic effect by its affinity for the sigma<html><sup>70</sup></html> subunit of the host RNA Polymerase causing misappropriation of the holoenzyme inhibiting transcription.<br> | In this gene construct, the T7 phage protein Gp2 is used as the gene of interest which has a bacteriostatic effect by its affinity for the sigma<html><sup>70</sup></html> subunit of the host RNA Polymerase causing misappropriation of the holoenzyme inhibiting transcription.<br> | ||
This part is used as a light-mediated growth regulatory system to dynamically control bacterial cell populations. | This part is used as a light-mediated growth regulatory system to dynamically control bacterial cell populations. | ||
Revision as of 12:15, 21 October 2019
CcaS-Gp2 + Ho1-PcyA-CcaR
This part consists of all the components of the CcaS-CcaR optogenetic system with the bacteriostatic growth-inhibitory protein Gp2 downstream of the pCpcG2 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2247
Illegal NheI site found at 2270
Illegal NheI site found at 3998
Illegal NheI site found at 4021 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
CcaSR is a green-light activated two-component signal transduction system (TCS) that controls the expression of phycobilisome linker protein CpcG2 in Synechocystis sp. PCC6803 complementary chromatic adaptation pathway. The naturally occurring CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. Upon activation with green light, the CcaS is autophosphorylated to a red-absorbing conformation that undergoes phosphotransfer to the response regulator CcaR leading to the activation of the pCpcG2 promoter initiating transcription of the gene of interest.
In this gene construct, the T7 phage protein Gp2 is used as the gene of interest which has a bacteriostatic effect by its affinity for the sigma70 subunit of the host RNA Polymerase causing misappropriation of the holoenzyme inhibiting transcription.
This part is used as a light-mediated growth regulatory system to dynamically control bacterial cell populations.