Low glucose environments cause the cAMP-CRP complex to bind target DNA and regulate hundreds of gene targets. [1] There are 3 classes of CRP promoters, categorized by the number and placement of CRP binding sites within each. Class I promoters have one CRP binding site at various distances upstream of the -35 box which interact with the α-C Terminal Domain (α-CTD) of RNA Polymerase (RNAP) . [16]Class II promoters have CRP encompassing the -35 box, and interact with α-CTD and α-NTD of RNAP for promoter recruiting .[ 17] This site partially occludes the σ70 subunit of RNAP, and is likely why multiple interactions with cAMP-CRP are made. Class III promoters contain a mix of multiple Class I and II CRP sites. [16]
+
PT-αcrp is a Glucose starved promoter，which is response to glucose starvation
−
Three versions of each promoter were generated: (1) “full length” promoters (crp) starting ~ 500 bp upstream of the open reading frame and ending at the transcription start site (2) minimal “truncated” promoters (T-crp) containing ~ 50 bp upstream of the transcription start site with care taken to include the full CRP binding site, and (3) enhanced truncated promoters (T-αcrp) which include a modified enhancer element from the CC(-41.5)α(-63) synthetic elements upstream of the T-crp promoters. 20
+
It can be used to Initiate gene expressing in response to glucose starvation.
−
+
−
'''what it is'''：Glucose starved promoter
+
−
+
−
'''what it does'''：Response to glucose starvation
+
−
+
−
'''how to use it in their projects'''：Initiating the autolysis gene expressing lactic acid bacteria in response to glucose starvation
+
−
+
<!-- Add more about the biology of this part here
<!-- Add more about the biology of this part here
Line 25:
Line 18:
<partinfo>BBa_K3142012 parameters</partinfo>
<partinfo>BBa_K3142012 parameters</partinfo>
<!-- -->
<!-- -->
−
==SZPT-China==
+
−
To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.
+
===Reference===
−
[[file:p-a.png|center|500px]]
+
*William Henry Bothfeld, Grace Kapov, and Keith Tyo. A glucose-sensing toggle switch for autonomous, high productivity genetic control. ACS Synth. 2017, 6(7):1296-1304.
Revision as of 07:56, 21 October 2019
Glucose starvation promoter（PT-αcrp）
PT-αcrp is a Glucose starved promoter，which is response to glucose starvation
It can be used to Initiate gene expressing in response to glucose starvation.
Sequence and Features
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 26
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 26
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 26 Illegal BamHI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 26
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 26
1000
COMPATIBLE WITH RFC[1000]
Reference
William Henry Bothfeld, Grace Kapov, and Keith Tyo. A glucose-sensing toggle switch for autonomous, high productivity genetic control. ACS Synth. 2017, 6(7):1296-1304.
