Difference between revisions of "Part:BBa K3142011:Design"

(→‎Design Notes)
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===References===
 
===References===
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Edgar Espinosa-Hernández, et al. The insertion of bioactive peptides at the C-terminal end of an 11S globulin changes the structural stability and improves the antihypertensive activity [J].Electronic Journal of Biotechnology2019, Vol. 37:18–24

Latest revision as of 01:19, 21 October 2019


Complex of 11S globulin from amaranth and hypotensive peptide multimer.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 217
    Illegal PstI site found at 443
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for lactic acid bacteria.

Source

References

Edgar Espinosa-Hernández, et al. The insertion of bioactive peptides at the C-terminal end of an 11S globulin changes the structural stability and improves the antihypertensive activity [J].Electronic Journal of Biotechnology2019, Vol. 37:18–24