Difference between revisions of "Part:BBa K3165033"
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<partinfo>BBa_K3165033 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3165033 SequenceAndFeatures</partinfo> | ||
Latest revision as of 00:42, 21 October 2019
RBS + ECFP
The codon optimized engineered cyan fluorescent protein (ECFP) is placed along with a ribosome binding site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Biology
Engineered Cyan Fluorescent protein is a commonly used reporter gene for biological studies. Owing to its photostability, sufficiently high lifespan and relatively low maturation times, ECFP is a popular choice as a fluorescent marker. ECFP paired with an RBS (BBa_B0034) is used to generate a translational unit for the production of the fluorophore.
Usage
This part is used as a reporter unit under any suitable promoter. Under a photo-sensitive promoter system, this part can be used to characterize the functioning of the inducible system based on fluorescence measurements.