Difference between revisions of "Part:BBa K3183001:Design"
Line 9: Line 9:
  
 
===Design Notes===
 
===Design Notes===
−
n/a
+
RFC 10, 12, 21, 23, 25, 1000 compatible.
  
  

Revision as of 21:19, 21 October 2019


Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into BBa_K3183102, which was finally assembled into the pTRKH3 vector BBa_K3183050, again by Gibson Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC 10, 12, 21, 23, 25, 1000 compatible.


Source

It is derived from the L.acidophilus surface layer protein promoter.

References

Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.