Difference between revisions of "Part:BBa K108029:Design"
Angel6767Q (Talk | contribs) |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K108029 short</partinfo> | <partinfo>BBa_K108029 short</partinfo> | ||
Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
− | pWW0 | + | XylS was cloned from pWW0 of Pseudomonas putida (reference 1 and 2). |
===References=== | ===References=== | ||
+ | 1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373. | ||
+ | |||
+ | 2. NCBI. |
Latest revision as of 08:48, 25 October 2008
XylS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 754
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 217
Design Notes
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
Source
XylS was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
References
1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.
2. NCBI.