Difference between revisions of "Part:BBa K108019:Design"
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__NOTOC__ | __NOTOC__ | ||
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===Source=== | ===Source=== | ||
− | pBHR68 | + | phbC was cloned from pBHR68 (reference 1). |
===References=== | ===References=== | ||
+ | 1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311. |
Latest revision as of 07:28, 25 October 2008
PhbC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 581
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 307
Illegal NgoMIV site found at 541
Illegal NgoMIV site found at 853
Illegal NgoMIV site found at 1132
Illegal NgoMIV site found at 1545
Illegal NgoMIV site found at 1612
Illegal AgeI site found at 280 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 and Not1 using site-directed mutation.
Source
phbC was cloned from pBHR68 (reference 1).
References
1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.