Difference between revisions of "Part:BBa K2940014"
Jakub Chromy (Talk | contribs) |
Jakub Chromy (Talk | contribs) |
||
| Line 13: | Line 13: | ||
The E. coli TOP10 cells transformed with XylR:sfGFP_pSB1C3 biosensor plasmid exhibited strong fluorescence even when uninduced. This strong background fluorescence was most presumably the result of sfGFP leaky expression and made cells apparently not sensitive to the induction when grown on plain and 10 mg/L toluene-containing LB agar plates (Figure 1). | The E. coli TOP10 cells transformed with XylR:sfGFP_pSB1C3 biosensor plasmid exhibited strong fluorescence even when uninduced. This strong background fluorescence was most presumably the result of sfGFP leaky expression and made cells apparently not sensitive to the induction when grown on plain and 10 mg/L toluene-containing LB agar plates (Figure 1). | ||
| − | [[T--Edinburgh OG-- | + | [[T--Edinburgh OG--Figure1 Jakub.png]] |
<!-- --> | <!-- --> | ||
Revision as of 22:20, 20 October 2019
XylR coding device-sfGFP_optimism coding device with Pu
This part encodes the biosensor genetic circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene. This part is a modification of the BBa_K2023015 part originally developed by Ionis Paris iGEM team 2016 [1]. The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.
Usage and Biology
BTEX (Benzene, Toluene, Ethylbenzene, Xylene)-like aromatic compounds detection.
Characterization
The E. coli TOP10 cells transformed with XylR:sfGFP_pSB1C3 biosensor plasmid exhibited strong fluorescence even when uninduced. This strong background fluorescence was most presumably the result of sfGFP leaky expression and made cells apparently not sensitive to the induction when grown on plain and 10 mg/L toluene-containing LB agar plates (Figure 1).
T--Edinburgh OG--Figure1 Jakub.png
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
Illegal XhoI site found at 3018 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]