Difference between revisions of "Part:BBa K2940014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400). | + | The part is identical to the BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400). |
| − | The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities. | + | The codon-optimized superfolder GFP variant was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities. However, the use of this strong GFP variant resulted in strongly constitutively fluorescent E. coli cells impeding the function of the biosensor. |
===Source=== | ===Source=== | ||
Revision as of 21:01, 20 October 2019
XylR coding device-sfGFP_optimism coding device with Pu
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is identical to the BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).
The codon-optimized superfolder GFP variant was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities. However, the use of this strong GFP variant resulted in strongly constitutively fluorescent E. coli cells impeding the function of the biosensor.
Source
References
[1] http://2016.igem.org/Team:Ionis_Paris