Difference between revisions of "Part:BBa K2940014:Design"
Jakub Chromy (Talk | contribs) (→Design Notes) |
Jakub Chromy (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
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The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400). | The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400). | ||
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The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities. | The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities. | ||
Revision as of 20:52, 20 October 2019
XylR coding device-sfGFP_optimism coding device with Pu
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
Illegal XhoI site found at 3018 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).
The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities.
Source
The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016, except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).